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Analytical Facilities
Wildlife DNA Services operates from the GLP/GXP compliant laboratories situated at Tepnel Scientific Services. These laboratories include a dedicated positive pressure DNA extraction laboratory, separate PCR preparation rooms and a sequencing laboratory containing, amongst other equipment, an IQOQ compliant ABI3130xl DNA sequencer. All data relating to the processing of samples is recorded through a custom-built genetic database, the Genetic Data Management System (GDMS). A fully implemented LIMS system is also in operation for all high-throughput genotyping.
Extraction Facility
Our extraction lab is designed to allow DNA recovery from forensic samples and low-yield project samples. The positive pressure room is maintained as a PCR-free environment to minimise contamination risk. We operate a strict non-transfer policy between labs for equipment and chemicals. All DNA extractions take place within a UV sterilized, HEPA filtered, laminar-flow fume cupboard.
Sequencing Facility
The second lab houses our genetic analysis equipment used for the wide range of DNA analysis applications available. Specific facilities include:
Applied Biosystems 3130xl capillary sequencer
MJ Research thermocycler suite
Applied Biosystems 7500 RT-PCR machine
Agilent 2100 Bioanalyzer
High-throughput Facility
The HT lab contains the Duncan thermocycler, associated liquid handling robotics and an Applied Biosystems 7900 RT-PCR machine that enables fully automated genotyping in 384-well plate format.
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